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SouthernBiotech collagen 2
Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
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Boster Bio collagen ii antibody
Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
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Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
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Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
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Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and <t>collagen</t> <t>2</t> staining ( C ). Scale bars equal 20 µm.
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Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and <t>collagen</t> <t>2</t> staining ( C ). Scale bars equal 20 µm.
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Bioss rabbit polyclonal anti type ii collagen
Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and <t>collagen</t> <t>2</t> staining ( C ). Scale bars equal 20 µm.
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Image Search Results


Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

Techniques: Immunohistochemical staining, Staining

Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

Techniques: Immunohistochemical staining, Staining

Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and collagen 2 staining ( C ). Scale bars equal 20 µm.

Journal: eLife

Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis

doi: 10.7554/eLife.103332

Figure Lengend Snippet: Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and collagen 2 staining ( C ). Scale bars equal 20 µm.

Article Snippet: Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight.

Techniques: Injection, Staining

The gating strategy to identify and sort CD47 Hi vs. CD47 Lo cell populations ( A–C ). CD47 Hi vs. CD47 Lo cells were identified and isolated from normal and osteoarthritis (OA) synovial tissue ( B ). Chondrogenic differentiation of CD47 Hi vs. CD47 Lo cells using pellet culture and stained with Alcian Blue ( D ). GAG quantification of the pellets ( E ). The pellets were also stained with Collagen 2 (Col2) as a marker of mature cartilage ECM ( E, G ). Signifiance determined by1 way ANOVA. p <0.05. Scale bars equal 100 µm.

Journal: eLife

Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis

doi: 10.7554/eLife.103332

Figure Lengend Snippet: The gating strategy to identify and sort CD47 Hi vs. CD47 Lo cell populations ( A–C ). CD47 Hi vs. CD47 Lo cells were identified and isolated from normal and osteoarthritis (OA) synovial tissue ( B ). Chondrogenic differentiation of CD47 Hi vs. CD47 Lo cells using pellet culture and stained with Alcian Blue ( D ). GAG quantification of the pellets ( E ). The pellets were also stained with Collagen 2 (Col2) as a marker of mature cartilage ECM ( E, G ). Signifiance determined by1 way ANOVA. p <0.05. Scale bars equal 100 µm.

Article Snippet: Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight.

Techniques: Isolation, Staining, Marker